Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation
Identifieur interne : 001B43 ( Main/Exploration ); précédent : 001B42; suivant : 001B44Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation
Auteurs : Zehava Faltin [Israël] ; Doron Holland [Israël] ; Margarita Velcheva [Canada] ; Marina Tsapovetsky [Israël] ; Patricia Roeckel-Drevet [France] ; Avtar K. Handa [États-Unis] ; Mohamad Abu-Abied [Israël] ; Miriam Friedman-Einat [Israël] ; Yuval Eshdat [Israël] ; Avihai Perl [Israël]Source :
- Plant and Cell Physiology [ 0032-0781 ] ; 2010-07.
English descriptors
- KwdEn :
- Citrus (enzymology), Gene Expression Regulation, Plant, Glutathione Peroxidase (metabolism), Homeostasis, Mutagenesis, Site-Directed, Oxidation-Reduction, Oxidative Stress, Plant Shoots (growth & development), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Reactive Oxygen Species (metabolism), Regeneration, Stress, Physiological, Tobacco (growth & development), Tobacco (metabolism), Transformation, Genetic.
- MESH :
- chemical , metabolism : Glutathione Peroxidase, Reactive Oxygen Species.
- enzymology : Citrus.
- growth & development : Plant Shoots, Plants, Genetically Modified, Tobacco.
- metabolism : Plants, Genetically Modified, Tobacco.
- mix :
Abstract
Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.
Url:
- https://api.istex.fr/document/A4D64ABF200EC222D26122C38E3F624D8E26005C/fulltext/pdf
- https://hal.archives-ouvertes.fr/hal-00964726
DOI: 10.1093/pcp/pcq082
Affiliations:
- Canada, France, Israël, États-Unis
- Auvergne (région administrative), Auvergne-Rhône-Alpes, Indiana
- Aubière
- Université Blaise-Pascal
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000A87
- to stream Istex, to step Curation: 000A87
- to stream Istex, to step Checkpoint: 000350
- to stream Hal, to step Corpus: 000010
- to stream Hal, to step Curation: 000010
- to stream Hal, to step Checkpoint: 000009
- to stream PubMed, to step Corpus: 000823
- to stream PubMed, to step Curation: 000823
- to stream PubMed, to step Checkpoint: 000823
- to stream Ncbi, to step Merge: 000B85
- to stream Ncbi, to step Curation: 000B85
- to stream Ncbi, to step Checkpoint: 000B85
- to stream Main, to step Merge: 001B81
- to stream Main, to step Curation: 001B43
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation</title><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name></author><author><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name></author><author><name sortKey="Velcheva, Margarita" sort="Velcheva, Margarita" uniqKey="Velcheva M" first="Margarita" last="Velcheva">Margarita Velcheva</name></author><author><name sortKey="Tsapovetsky, Marina" sort="Tsapovetsky, Marina" uniqKey="Tsapovetsky M" first="Marina" last="Tsapovetsky">Marina Tsapovetsky</name></author><author><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name></author><author><name sortKey="Handa, Avtar K" sort="Handa, Avtar K" uniqKey="Handa A" first="Avtar K." last="Handa">Avtar K. Handa</name></author><author><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A4D64ABF200EC222D26122C38E3F624D8E26005C</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1093/pcp/pcq082</idno><idno type="url">https://api.istex.fr/document/A4D64ABF200EC222D26122C38E3F624D8E26005C/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A87</idno><idno type="wicri:Area/Istex/Curation">000A87</idno><idno type="wicri:Area/Istex/Checkpoint">000350</idno><idno type="wicri:doubleKey">0032-0781:2010:Faltin Z:glutathione:peroxidase:regulation</idno><idno type="wicri:source">HAL</idno><idno type="RBID">Hal:hal-00964726</idno><idno type="url">https://hal.archives-ouvertes.fr/hal-00964726</idno><idno type="wicri:Area/Hal/Corpus">000010</idno><idno type="wicri:Area/Hal/Curation">000010</idno><idno type="wicri:Area/Hal/Checkpoint">000009</idno><idno type="wicri:doubleKey">0032-0781:2010:Faltin Z:glutathione:peroxidase:regulation</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:20530511</idno><idno type="wicri:Area/PubMed/Corpus">000823</idno><idno type="wicri:Area/PubMed/Curation">000823</idno><idno type="wicri:Area/PubMed/Checkpoint">000823</idno><idno type="wicri:Area/Ncbi/Merge">000B85</idno><idno type="wicri:Area/Ncbi/Curation">000B85</idno><idno type="wicri:Area/Ncbi/Checkpoint">000B85</idno><idno type="wicri:Area/Main/Merge">001B81</idno><idno type="wicri:Area/Main/Curation">001B43</idno><idno type="wicri:Area/Main/Exploration">001B43</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation</title><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Velcheva, Margarita" sort="Velcheva, Margarita" uniqKey="Velcheva M" first="Margarita" last="Velcheva">Margarita Velcheva</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Alberta Research Council, PO Bag 4000, Hwy 16A and 75 Street, Vegreville, AB, T9C 1T4</wicri:regionArea><wicri:noRegion>T9C 1T4</wicri:noRegion></affiliation></author><author><name sortKey="Tsapovetsky, Marina" sort="Tsapovetsky, Marina" uniqKey="Tsapovetsky M" first="Marina" last="Tsapovetsky">Marina Tsapovetsky</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>UMR INRA 547-PIAF, Université Blaise Pascal, 63177 Aubière Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Aubière</settlement></placeName><orgName type="university">Université Blaise-Pascal</orgName></affiliation></author><author><name sortKey="Handa, Avtar K" sort="Handa, Avtar K" uniqKey="Handa A" first="Avtar K." last="Handa">Avtar K. Handa</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Indiana</region></placeName><wicri:cityArea>Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Dr., West Lafayette</wicri:cityArea></affiliation></author><author><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Animal Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Present address: The Chief Scientist of Israel Ministry of Agriculture & Rural Development, PO Box 30, 50250 Bet-Dagan</wicri:regionArea><wicri:noRegion>50250 Bet-Dagan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Israël</country></affiliation></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant and Cell Physiology</title><idno type="ISSN">0032-0781</idno><idno type="eISSN">1471-9053</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2010-07">2010-07</date><biblScope unit="volume">51</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1151">1151</biblScope><biblScope unit="page" to="1162">1162</biblScope></imprint><idno type="ISSN">0032-0781</idno></series><idno type="istex">A4D64ABF200EC222D26122C38E3F624D8E26005C</idno><idno type="DOI">10.1093/pcp/pcq082</idno><idno type="ArticleID">pcq082</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0032-0781</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus (enzymology)</term><term>Gene Expression Regulation, Plant</term><term>Glutathione Peroxidase (metabolism)</term><term>Homeostasis</term><term>Mutagenesis, Site-Directed</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term><term>Plant Shoots (growth & development)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Regeneration</term><term>Stress, Physiological</term><term>Tobacco (growth & development)</term><term>Tobacco (metabolism)</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Peroxidase</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Shoots</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="mix" xml:lang="en"><term>GLUTATHIONE PEROXIDASE</term><term>Gene Expression Regulation, Plant</term><term>Homeostasis</term><term>Mutagenesis, Site-Directed</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term><term>REACTIVE OXYGEN SPECIES</term><term>REDOC REGULATION</term><term>Regeneration</term><term>SHOOT REGENERATION</term><term>Stress, Physiological</term><term>TRANSGENIC PLANTS</term><term>Transformation, Genetic</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</div></front></TEI><affiliations><list><country><li>Canada</li><li>France</li><li>Israël</li><li>États-Unis</li></country><region><li>Auvergne (région administrative)</li><li>Auvergne-Rhône-Alpes</li><li>Indiana</li></region><settlement><li>Aubière</li></settlement><orgName><li>Université Blaise-Pascal</li></orgName></list><tree><country name="Israël"><noRegion><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name></noRegion><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name><name sortKey="Tsapovetsky, Marina" sort="Tsapovetsky, Marina" uniqKey="Tsapovetsky M" first="Marina" last="Tsapovetsky">Marina Tsapovetsky</name></country><country name="Canada"><noRegion><name sortKey="Velcheva, Margarita" sort="Velcheva, Margarita" uniqKey="Velcheva M" first="Margarita" last="Velcheva">Margarita Velcheva</name></noRegion></country><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name></region></country><country name="États-Unis"><region name="Indiana"><name sortKey="Handa, Avtar K" sort="Handa, Avtar K" uniqKey="Handa A" first="Avtar K." last="Handa">Avtar K. Handa</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B43 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001B43 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:A4D64ABF200EC222D26122C38E3F624D8E26005C
|texte= Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation
}}
| This area was generated with Dilib version V0.6.25. |  |